FACSAria IIA (Location: Dental School, room 441).Symphony A3 (Location: Dental School, Room 426).The laser wavelengths are 355nm (UV), 407nm (violet), 488nm (blue), 561nm (yellow-green), 633nm (red). Facility hardware: All A3 analyzers have been configured with 5 lasers and 25 detectors (23 colors + 2 scatter) sorters have been configured with 5 lasers and 19 detectors (17 colors + 2 scatter). Support in the form of training, technical assistance, troubleshooting and consultation are given to various laboratories that independently own cytometers on campus. The Emory University School of Medicine Core Facility for Flow Cytometry is located in the Dental School Building. If you need additional information please contact the Core Director. ) Facility Descriptionĭetailed facility information is given below.
Sort protocol: high-speed two-step sortġ.Sort goal: to use the sorted cells to make RNA for gene chip analysis.Sort criteria: separate out CD62L hi and lo populations from DNA- immunized mice 62 days post-vaccination.Source material: P14 chimeras (B6 mice initially transferred with 106 P14 splenocytes) and then immunized with DNA.In order to avoid the common pitfalls inevitable in preparing cells for sorting, we recommend reading the following links: DSB LSRII panel worksheet (special thanks to Suzanne Mertens and the EVC Flow Cytometry Core Facility).Post-Training Tutorial - Analog Analyzers.Post-Training Tutorial - Digital Analyzers.Cell-cycle protocol for yeast (special Thanks to Missy Brykailo and the Fridovich-Keil Lab).Intracellular Staining prep (special Thanks to Hilary Rosenthal, MT (ASCP) and the Waller Lab).Extracellular Immunophenotyping prep (special Thanks to Hilary Rosenthal, MT (ASCP) and the Waller Lab).Titering Directly Conjugated Antibodies to Extracellular Antigens (special Thanks to Dr.Determining Antibody Titers (special Thanks to Dr.
Flowjo tutorial compensation plus#
Flowjo tutorial compensation how to#
How to connect to the Flow Core server for data transfer Then fire up FlowJo (make sure you've registered FlowJo!) Let us know if you have any questions. How to use FlowJo off-campus using an Emory VPN connectionįollow these instructions to create and open a VPN (Virtual Private Network) connection to the Emory system.
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